Name：COVID-19 Nucleic Acid
RT-PCR Test Kit
Sample：throat swabs, nasal swabs,
nasopharyngeal, and oropharyngeal swabs specimens
Principles of the
The COVID-19 Nucleic
Acid RT-PCR Test Kit is a real-time reverse transcription polymerase chain
reaction (rRT -PCR) test. The COVID-19 primer and probe set(s) is designed to
detect RNA from the COVID-19 in throat swabs, nasal swabs, nasopharyngeal, and
oropharyngeal swabs specimens from patients with signs and symptoms of
infection who are suspected of COVID-19.
This kit uses
specific primers and TaqMan probes (5’ end-labeled FAM, VIC or ROX fluorescent
probes) to simultaneously amplify the COVID-19 ORF1ab gene, N gene fragments,
and actin gene in one tube. In the case where the COVID-19 RNA template is
contained in the reaction system, a fluorescent signal is released during the
PCR amplification process. The instrument is used to monitor the signal
intensity of the corresponding channel in real time during the PCR process to
achieve qualitative detection results.
This kit uses dUTP
and uracil-DNA N-glycosylase (UNG) to minimize PCR cross-contamination from
previously amplified PCR products. To avoid possible false positive results,
the reaction mixture undergoes a 2 min incubation at 37 °C prior to PCR amplification, allowing UNG
to fully degrade potential PCR product contamination.
The kit uses the human β-actin gene as an internal
reference gene. This internal control is detected using specific primers and
probes (5’end-labeled ROX fluorescent probes) in order to exclude false
negative results via confirming the success of the reaction in real time.